Dos Santos Ferreira MC, Stephens TG, Brinsmade SR. In Staphylococcus aureus, MbcS is a refunctionalized acyl-CoA synthetase that confers a fitness advantage during intra-species competition. bioRxiv [Preprint]. 2026 Mar 29:2026.03.28.714991. doi: 10.64898/2026.03.28.714991. PMID: 41928966; PMCID: PMC13041820.

DiMaggio DA Jr, Yeo W-S, Brinsmade SR. Pyrimidine sufficiency is required for Sae two-component system signaling in Staphylococcus aureus. J Bacteriol. 2025 Aug 21;207(8):e0011525. doi: 10.1128/jb.00115-25. Epub 2025 Jul 21. PMID: 40689635; PMCID: PMC12369341.

Alqahtani S, DiMaggio DA Jr, Brinsmade SR. CodY controls the SaeR/S two-component system by modulating branched-chain fatty acid synthesis in Staphylococcus aureus. J Bacteriol. 2024 Nov 21;206(11):e0019124. doi: 10.1128/jb.00191-24. Epub 2024 Oct 9. PMID: 39382300; PMCID: PMC11580410.

Dufresne K, DiMaggio DA Jr, Maduta CS, Brinsmade SR, McCormick JK. Discovery of an antivirulence compound that targets the Staphylococcus aureus SaeRS two-component system to inhibit toxic shock syndrome toxin-1 production. J Biol Chem. 2024 Jul;300(7):107455. doi: 10.1016/j.jbc.2024.107455. Epub 2024 Jun 7. PMID: 38852884; PMCID: PMC11328871.

Dos Santos Ferreira MC, Pendleton A, Yeo WS, Málaga Gadea FC, Camelo D, McGuire M, Brinsmade SR. In Staphylococcus aureus, the acyl-CoA synthetase MbcS supports branched-chain fatty acid synthesis from carboxylic acid and aldehyde precursors. Mol Microbiol. 2024 May;121(5):865-881. doi: 10.1111/mmi.15237. Epub 2024 Feb 16. PMID: 38366323; PMCID: PMC11167679.

Yeo WS, Dyzenhaus S, Torres VJ, Brinsmade SR, Bae T. Regulation of Bacterial Two-Component Systems by Cardiolipin. Infect Immun. 2023 Apr 18;91(4):e0004623. doi: 10.1128/iai.00046-23. Epub 2023 Mar 28. PMID: 36975788; PMCID: PMC10112254.

Pendleton A, Yeo WS, Alqahtani S, DiMaggio DA Jr, Stone CJ, Li Z, Singh VK, Montgomery CP, Bae T, Brinsmade SR. Regulation of the Sae Two-Component System by Branched-Chain Fatty Acids in Staphylococcus aureus. mBio. 2022 Oct 26;13(5):e0147222. doi: 10.1128/mbio.01472-22. Epub 2022 Sep 22. PMID: 36135382; PMCID: PMC9600363.

King AN, de Mets F, Brinsmade SR. Who's in control? Regulation of metabolism and pathogenesis in space and time. Curr Opin Microbiol. 2020 Jun;55:88-96. doi: 10.1016/j.mib.2020.05.009. Epub 2020 Jun 9. PMID: 32532689; PMCID: PMC7323893.

Collins MM, Behera RK, Pallister KB, Evans TJ, Burroughs O, Flack C, Guerra FE, Pullman W, Cone B, Dankoff JG, Nygaard TK, Brinsmade SR, Voyich JM. The Accessory Gene saeP of the SaeR/S Two-Component Gene Regulatory System Impacts Staphylococcus aureus Virulence During Neutrophil Interaction. Front Microbiol. 2020 Apr 22;11:561. doi: 10.3389/fmicb.2020.00561. PMID: 32390958; PMCID: PMC7189620.

Mlynek KD, Bulock LL, Stone CJ, Curran LJ, Sadykov MR, Bayles KW, Brinsmade SR. Genetic and Biochemical Analysis of CodY-Mediated Cell Aggregation in Staphylococcus aureus Reveals an Interaction between Extracellular DNA and Polysaccharide in the Extracellular Matrix. J Bacteriol. 2020 Mar 26;202(8):e00593-19. doi: 10.1128/JB.00593-19. PMID: 32015143; PMCID: PMC7099133.

Augagneur Y, King AN, Germain-Amiot N, Sassi M, Fitzgerald JW, Sahukhal GS, Elasri MO, Felden B, Brinsmade SR. Analysis of the CodY RNome reveals RsaD as a stress-responsive riboregulator of overflow metabolism in Staphylococcus aureus. Mol Microbiol. 2020 Feb;113(2):309-325. doi: 10.1111/mmi.14418. Epub 2019 Dec 16. PMID: 31696578.

Behera RK, Mlynek KD, Linz MS, Brinsmade SR. A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues. J Vis Exp. 2019 Feb 19;(144):10.3791/59055. doi: 10.3791/59055. PMID: 30855576; PMCID: PMC7295204.

King AN, Borkar SA, Samuels DJ, Batz Z, Bulock LL, Sadykov MR, Bayles KW, Brinsmade SR. Guanine Limitation Results in CodY-Dependent and -Independent Alteration of Staphylococcus aureus Physiology and Gene Expression. J Bacteriol. 2018 Jun 25;200(14):e00136-18. doi: 10.1128/JB.00136-18. PMID: 29712876; PMCID: PMC6018361.

Mlynek KD, Sause WE, Moormeier DE, Sadykov MR, Hill KR, Torres VJ, Bayles KW, Brinsmade SR. Nutritional Regulation of the Sae Two-Component System by CodY in Staphylococcus aureus. J Bacteriol. 2018 Mar 26;200(8):e00012-18. doi: 10.1128/JB.00012-18. PMID: 29378891; PMCID: PMC5869476.

Kaiser JC, King AN, Grigg JC, Sheldon JR, Edgell DR, Murphy MEP, Brinsmade SR, Heinrichs DE. Repression of branched-chain amino acid synthesis in Staphylococcus aureus is mediated by isoleucine via CodY, and by a leucine-rich attenuator peptide. PLoS Genet. 2018 Jan 22;14(1):e1007159. doi: 10.1371/journal.pgen.1007159. PMID: 29357354; PMCID: PMC5794164.

Samuels DJ, Wang Z, Rhee KY, Brinsmade SR. A Tandem Liquid Chromatography-Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus. J Vis Exp. 2017 Mar 28;(121):55558. doi: 10.3791/55558. PMID: 28448019; PMCID: PMC5564428.

Brinsmade SR. CodY, a master integrator of metabolism and virulence in Gram-positive bacteria. Curr Genet. 2017 Jun;63(3):417-425. doi: 10.1007/s00294-016-0656-5. Epub 2016 Oct 15. PMID: 27744611.

Waters NR, Samuels DJ, Behera RK, Livny J, Rhee KY, Sadykov MR, Brinsmade SR. A spectrum of CodY activities drives metabolic reorganization and virulence gene expression in Staphylococcus aureus. Mol Microbiol. 2016 Aug;101(3):495-514. doi: 10.1111/mmi.13404. Epub 2016 Jun 10. PMID: 27116338; PMCID: PMC5007081.

Belitsky BR, Brinsmade SR, Sonenshein AL. Intermediate Levels of Bacillus subtilis CodY Activity Are Required for Derepression of the Branched-Chain Amino Acid Permease, BraB. PLoS Genet. 2015 Oct 16;11(10):e1005600. doi: 10.1371/journal.pgen.1005600. PMID: 26473603; PMCID: PMC4608796.

Sassi M, Sharma D, Brinsmade SR, Felden B, Augagneur Y. Genome Sequence of the Clinical Isolate Staphylococcus aureus subsp. aureus Strain UAMS-1. Genome Announc. 2015 Feb 12;3(1):e01584-14. doi: 10.1128/genomeA.01584-14. PMID: 25676774; PMCID: PMC4333674.

Brinsmade SR, Alexander EL, Livny J, Stettner AI, Segrè D, Rhee KY, Sonenshein AL. Hierarchical expression of genes controlled by the Bacillus subtilis global regulatory protein CodY. Proc Natl Acad Sci U S A. 2014 Jun 3;111(22):8227-32. doi: 10.1073/pnas.1321308111. Epub 2014 May 19. PMID: 24843172; PMCID: PMC4050614.

Kriel A, Brinsmade SR, Tse JL, Tehranchi AK, Bittner AN, Sonenshein AL, Wang JD. GTP dysregulation in Bacillus subtilis cells lacking (p)ppGpp results in phenotypic amino acid auxotrophy and failure to adapt to nutrient downshift and regulate biosynthesis genes. J Bacteriol. 2014 Jan;196(1):189-201. doi: 10.1128/JB.00918-13. Epub 2013 Oct 25. PMID: 24163341; PMCID: PMC3911124.

Brinsmade SR, Sonenshein AL. Dissecting complex metabolic integration provides direct genetic evidence for CodY activation by guanine nucleotides. J Bacteriol. 2011 Oct;193(20):5637-48. doi: 10.1128/JB.05510-11. Epub 2011 Aug 19. PMID: 21856856; PMCID: PMC3187194.

Lima BP, Antelmann H, Gronau K, Chi BK, Becher D, Brinsmade SR, Wolfe AJ. Involvement of protein acetylation in glucose-induced transcription of a stress-responsive promoter. Mol Microbiol. 2011 Sep;81(5):1190-204. doi: 10.1111/j.1365-2958.2011.07742.x. Epub 2011 Jul 28. PMID: 21696463; PMCID: PMC3180887.

Brinsmade SR, Kleijn RJ, Sauer U, Sonenshein AL. Regulation of CodY activity through modulation of intracellular branched-chain amino acid pools. J Bacteriol. 2010 Dec;192(24):6357-68. doi: 10.1128/JB.00937-10. Epub 2010 Oct 8. PMID: 20935095; PMCID: PMC3008522.

Brinsmade SR, Escalante-Semerena JC. In vivo and in vitro analyses of single-amino acid variants of the Salmonella enterica phosphotransacetylase enzyme provide insights into the function of its N-terminal domain. J Biol Chem. 2007 Apr 27;282(17):12629-40. doi: 10.1074/jbc.M611439200. Epub 2007 Mar 5. Erratum in: J Biol Chem. 2007 Jun 1;282(22):16712. PMID: 17339319.

Kim YR, Brinsmade SR, Yang Z, Escalante-Semerena J, Fierer J. Mutation of phosphotransacetylase but not isocitrate lyase reduces the virulence of Salmonella enterica serovar Typhimurium in mice. Infect Immun. 2006 Apr;74(4):2498-502. doi: 10.1128/IAI.74.4.2498-2502.2006. PMID: 16552088; PMCID: PMC1418904.

Brinsmade SR, Paldon T, Escalante-Semerena JC. Minimal functions and physiological conditions required for growth of Salmonella enterica on ethanolamine in the absence of the metabolosome. J Bacteriol. 2005 Dec;187(23):8039-46. doi: 10.1128/JB.187.23.8039-8046.2005. PMID: 16291677; PMCID: PMC1291257.

Brinsmade SR, Escalante-Semerena JC. The eutD gene of Salmonella enterica encodes a protein with phosphotransacetylase enzyme activity. J Bacteriol. 2004 Mar;186(6):1890-2. doi: 10.1128/JB.186.6.1890-1892.2004. PMID: 14996820; PMCID: PMC355984.

Silvaggi NR, Anderson JW, Brinsmade SR, Pratt RF, Kelly JA. The crystal structure of phosphonate-inhibited D-Ala-D-Ala peptidase reveals an analogue of a tetrahedral transition state. Biochemistry. 2003 Feb 11;42(5):1199-208. doi: 10.1021/bi0268955. PMID: 12564922.