Publications

Pendleton A, Yeo W-S, Alqahtani S, DiMaggio Jr DA, Stone CJ, Li Z, Singh VK, Montgomery CP, Bae T, Brinsmade SR. Regulation of the Sae Two-Component System by Branched-Chain Fatty Acids in Staphylococcus aureus mBio. 2022. (), e0147222
Augagneur Y*, King AN*, Germain-Amiot N, Sassi M, Fitzgerald FW, Sahukhal GS, Elasri MO, Felden B, Brinsmade SR. Analysis of the CodY RNome reveals RsaD as a stress-responsive riboregulator of overflow metabolism in Staphylococcus aureus Mol Microbiol 2020. 113(2), 1-17
King AN*, de Mets F*, Brinsmade SR. Who’s in control? Regulation of metabolism and pathogenesis in space and time Curr Opin Microbiol. 2020. 55(), 88-96
Behera RK, Mlynek KD, Linz MS, Brinsmade SR. A fluorescence-based method to study bacterial gene regulation in infected tissues J Vis Exp. 2019. 144(), e59055
Mlynek KD, Bulock LL, Stone CJ, Curran LJ, Sadykov MR, Bayles KW, and Brinsmade SR.. Genetic and biochemical analysis of CodY-mediated cell aggregation in Staphylococcus aureus reveals an interaction between eDNA and polysaccharide in the extracellular matrix J Bacteriol. 2020. 202(8), e00593-19
King AN, Borkar SA, Samuels DJ, Batz Z, Bulock LL, Sadykov MR, Bayles KW, Brinsmade SR. Guanine limitation results in CodY-dependent and -independent alteration of Staphylococcus aureus physiology and gene expression J Bacteriol. 2018. (), pii: JB.00136-18
Collins MM, Behera RK, Pallister KB, Evans TJ, Burroughs O, Flack C, Guerra FE, Pullman W, Brock Cone, Dankoff JG, Nygaard TK, Brinsmade SR, Voyich JM. The Accessory Gene saeP of the SaeR/S Two-Component Gene Regulatory System Impacts Staphylococcus aureus Virulence During Neutrophil Interaction Front Microbiol. 2020. 11(), 561
Mlynek KD, Sause WE, Moormeier DE, Sadykov MR, Hill KR, Torres VJ, Bayles KW, Brinsmade SR. Nutritional regulation of the Sae two-component system by CodY in Staphylococcus aureus J Bacteriol. 2018. 200(8), pii: e00012-18
Kaiser JC, King AN, Grigg JC, Sheldon JR, Edgell DR, Murphy MEP, Brinsmade SR, Heinrichs DE. Repression of branched-chain amino acid synthesis in Staphylococcus aureus is mediated by isoleucine via CodY, and by a leucine-rich attenuator peptide. PLoS Genet. 2018. 14(1), e1007159
Samuels DJ, Wang Z, Rhee KY, Brinsmade SR. A Tandem Liquid Chromatography-Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus J Vis Exp. 2017. 121(),
Brinsmade, SR. CodY, a master integrator of metabolism and virulence in Gram-positive bacteria Curr Genet. 2017. 63(3), 417-425
Waters NR, Samuels DJ, Behera RK, Livny J, Rhee KY, Sadykov MR, Brinsmade SR. A Spectrum of CodY Activities Drives Metabolic Reorganization and Virulence Gene Expression in Staphylococcus aureus. Mol Microbiol 2016. 101(3), 495-514
Sassi M, Sharma D, Brinsmade SR, Felden B, Augagneur Y. Genome sequence of the clinical isolate Staphylococcus aureus subsp. aureus Strain UAMS-1. Genome Announc. 2015. 3(1), e01584-14
Belitsky BR, Brinsmade SR, Sonenshein AL. Intermediate levels of Bacillus subtilis CodY activity are required for derepression of the branched-chain amino acid permease, BraB. PLoS Genetics 2015. 11(10), e1005600
Brinsmade SR, Alexander EL, Livny J, Stettner AI, Segrè D, Rhee KY, Sonenshein AL. Hierarchical expression of genes controlled by the Bacillus subtilis global regulatory protein CodY. Proc Natl Acad Sci U S A. 2014. 111(22), 8227-8232
Kriel A, Brinsmade SR, Tse JL, Tehranchi AK, Bittner AN, Sonenshein AL, Wang JD. GTP dysregulation in Bacillus subtilis cells lacking (p)ppGpp results in phenotypic amino acid auxotrophy and failure to adapt to nutrient downshift and regulate biosynthesis genes. J. Bacteriol. 2014. 196(1), 189-201
Lima BP, Antelmann H, Gronau K, Chi BK, Becher D, Brinsmade SR, Wolfe AJ. Involvement of protein acetylation in glucose-induced transcription of a stress-responsive promoter. Mol. Microbiol. 2011. 81(5), 1190-204
Brinsmade SR, Sonenshein AL. Dissecting complex metabolic integration provides direct genetic evidence for CodY activation by guanine nucleotides. J. Bacteriol. 2011. 193(20), 5637-48
Brinsmade SR, Kleijn RJ, Sauer U, Sonenshein AL. Regulation of CodY activity through modulation of intracellular branched-chain amino acid pools. J. Bacteriol. 2010. 192(24), 6357-68
Brinsmade SR, Escalante-Semerena JC. In vivo and in vitro analyses of single-amino acid variants of the Salmonella enterica phosphotransacetylase enzyme provide insights into the function of its N-terminal domain. J. Biol. Chem. 2007. 282(17), 12629-40
Kim YR, Brinsmade SR, Yang Z, Escalante-Semerena J, Fierer J. Mutation of phosphotransacetylase but not isocitrate lyase reduces the virulence of Salmonella enterica serovar Typhimurium in mice. Infect. Immun. 2006. 74(4), 2498-502
Brinsmade SR, Paldon T, Escalante-Semerena JC. Minimal functions and physiological conditions required for growth of Salmonella enterica on ethanolamine in the absence of the metabolosome. J. Bacteriol. 2005. 187(23), 8039-46
Brinsmade SR, Escalante-Semerena JC. The eutD gene of Salmonella enterica encodes a protein with phosphotransacetylase enzyme activity. J. Bacteriol. 2004. 186(6), 1890-2
Silvaggi NR, Anderson JW, Brinsmade SR, Pratt RF, Kelly JA. The crystal structure of phosphonate-inhibited D-Ala-D-Ala peptidase reveals an analogue of a tetrahedral transition state. Biochemistry 2003. 42(5), 1199-208